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Histochemical staining of DNA, RNA, protein, and starch were observed during organogenesis in Gladiolus ´Topaz´ callus. Staining intensities of DNA, RNA, and protein were higher at near epidermal tissues which showed increased cell division activity in subculturing callus. In the organogenic callus, DNA, RNA, and protein were stained more intensively at organ-forming regions. In contrast to DNA, RNA, and protein, starch showed higher intensity at surrounding tissue of organ-forming regions and higher amount of starch was accumulated at shoot forming callus than root forming callus.
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